Figure 2.

General application of the proteinase K digestion (P), EDTA autoclaving (A), and formic acid (FA) treatment (F) (in that order; referred to as “PAF”) method in amyloid β peptide (Aβ) immunohistochemistry. Serial (A–B, C–D, E–F, G–H) brain tissue sections from a 74-year-old male patient with Alzheimer disease (the same patient as shown in Fig. 1) (A–D) and a 16-month-old (E, F) and a 15-month-old (G, H) amyloid precursor protein–Swedish/London (APP-SL) mouse of line 7–5 were immunostained with monoclonal anti-Aβ antibodies 6E10 (A, B, E, F) and 4G8 (C, D, G, H) following pretreatment by the FA (A, C, E, G) and PAF methods (B, D, F, H). Pictures are from the cingulate cortex (A, B) and the frontal cortex (C, D). Scale bar = 200 µm (A–H).