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. 2012 Nov;60(11):844–853. doi: 10.1369/0022155412459759

Figure 3.

Figure 3.

Representative examples of Acr-dG immunofluorescence in BEAS-2B cells treated with varying concentrations of Acr. BEAS-2B cells were treated with Acr at varying concentrations for 24 hr as described in the Materials and Methods. Immunofluorescence was performed using an anti-Acr-dG antibody. (A) 4′,6-Diamidino-2-phenylindole (DAPI) channel image of BEAS-2B cells, no Acr treatment. (B) Alexa Fluor 488 channel image of BEAS-2B cells, no Acr treatment. (C) DAPI channel image of BEAS-2B cells, 20 µM Acr treatment. (D) FITC channel image of BEAS-2B cells, 20 µM Acr treatment. (E) DAPI channel image of BEAS-2B cells, 35 µM Acr treatment. (F) FITC channel image of BEAS-2B cells, 35 µM Acr treatment. (G) DAPI channel image of BEAS-2B cells, 50 µM Acr treatment. (H) FITC channel image of BEAS-2B cells, 50 µM Acr treatment. (I) DAPI channel image of BEAS-2B cells, 100 µM Acr treatment. (J) FITC channel image of BEAS-2B cells, 100 µM Acr treatment. (K) DAPI channel image of BEAS-2B cells, 200 µM Acr treatment. (L) FITC channel image of BEAS-2B cells, 200 µM Acr treatment. The scale bar size is 100 µm.