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Liquid chromatography/tandem mass spectrometry/multiple-reaction monitoring (LC-MS/MS-MRM) analysis of Acr-dG levels in BEAS-2B cells. BEAS-2B cells were treated with varying concentrations of Acr for 24 hr. DNA was extracted from cells and LC-MS/MS-MRM analysis of Acr-dG levels in DNA was performed as described in the Materials and Methods. The experiment was performed in duplicate for each concentration of Acr.
