Figure 6.

Acr-dG immunofluorescence in human oral cells. Human oral cells from a smoker were subjected to immunofluorescence staining using an antibody against Acr-dG as described in the Materials and Methods. (A) 4′,6-Diamidino-2-phenylindole (DAPI) channel image indicating the location of the nuclei. (B) Acr-dG immunofluorescence (Cy3). Arrows indicate nuclei that are positive for Acr-dG adducts. (C) Negative control for Acr-dG immunofluorescence (Cy3). The procedure remained the same except for no incubation with Acr-dG antibody. Note that although there is background fluorescence, there is no nuclear staining. The scale bar size is 100 µm.