Figure 7.

akt-1 and daf-2 function from different tissues to regulate damage-induced germ cell apoptosis. (a) Young adult worms were transferred to fresh RNAi plates, treated with IR and germ cell apoptosis was quantified after 24 h at 20°C. rrf-1 and ppw-1 are required for RNAi in the soma and germline, respectively. rrf-1(0)=rrf-1(pk1417), ppw-1(lf)=ppw-1(pk1425). Error bars as in Figure 1. *P<0.05 versus control(RNAi). (b) L4-stage worms were transferred to fresh RNAi plates, treated with IR and germ cell apoptosis was quantified after 24 h at 20°C. Error bars as in Figure 1. *P<0.01 versus control(RNAi). (c) Young adult worms were treated with IR and germ cell apoptosis was quantified after 24 h at 20°C. byEx[akt-1(+)] is a high-copy extrachromosomal array that rescues the somatic Daf-c phenotype of akt-1(ok525); akt-2(RNAi) worms. akt-1(0)=akt-1(ok525). Error bars are as in Figure 1. *P<0.05 versus wild type. (d) L4-stage worms were transferred to fresh plates, treated with IR and germ cell apoptosis was quantified after 24 h at 20°C. daf-2(lf)=daf-2(e1370), hpEx[P_F25B3.3daf-2(+)]=hpEx792, a high-copy array that expresses daf-2 in the nervous system; hpEx[P_myo-3daf-2(+)]=hpEx791, a high-copy array that expresses daf-2 in muscle. Error bars are as in Figure 1