Figure 2.

Oxidative-mediated OPA1 desoligomerization following complex I inhibition. (a) Isolated nonsynaptosomal mouse brain mitochondria treated with different doses of MPP⁺ or osmotically swollen for 15 min were incubated with 1 mM (EDC) for 30 min followed by centrifugation. Proteins in the pellet were separated by SDS-PAGE and immunoblotted using anti-OPA1 antibodies. The asterisk indicates OPA1 oligomer; arrowheads indicate non-oligomeric OPA1. Histograms represent average quantification of OPA1 oligomers±S.E.M. from at least three independent experiments. (b) Isolated nonsynaptosomal mouse brain mitochondria were treated, processed and quantified as in a, in the presence or absence of tempol (500 μM for 15 min). In (a and b), *P<0.05, compared with untreated mitochondria; in b, ^#P<0.05, compared with MPP⁺-treated, tempol-free mitochondria