Figure 3.

DΔNp53 induced wg expression in undead cells. (a–f) Wg protein was stained with an anti-Wg antibody (green). (a, b) The wg expression in wild-type (a) and in p53-null wing discs (b). (c and d) Double staining Wg (green) and RFP (red). Overproduction of Dp53 or DΔNp53 in wing imaginal discs expressing p35 and RFP (en>p35>RFP). The engrailed domain expression is visualized by RFP. Wg (c′ and d′) and RFP (c″ and d″) single fluorescent channels are shown. (c, c′ and c″) A mild induction of Wg is induced by Dp53 overproduction (en>Dp53) resulting in broadening of the endogenous Wg expression pattern in the engrailed domain. (d, d′ and d″) The overproduction of DΔNp53 (en>DΔNp53) induces a strong and widespread induction of Wg inside and outside the engrailed domain of expression (arrow). (e and f) Overproduction of Dp53 or DΔNp53 in wing imaginal discs mutant for dronc^I29. (e) A mild induction of Wg is induced by Dp53 overproduction (en>Dp53; dronc^I29−/−) resulting in broadening of the endogenous Wg expression pattern in the engrailed domain. (f) The overproduction of DΔNp53 (en>DΔNp53; dronc^I29−/−) induces a strong and widespread induction of Wg. Scale bars are 100 μm