Figure 4.

DΔNp53 induced wg expression and enhanced proliferation in wing imaginal discs. (a, d and g) Control wing imaginal discs (MS1096>LacZ). (b, e and h) Wing imaginal discs overproducing Dp53 (MS1096>Dp53). (c, f and i) Wing imaginal discs overproducing DΔNp53 (MS1096>DΔNp53). (a–c) Wg protein was stained with an anti-Wg antibody. (d–f) Actin was stained with phalloidin coupled with TRITC. (g–i) GFP fluorescence in wing imaginal discs carrying PCNA-EmGFP. (c) The overproduction of DΔNp53 leads to a strong increase of wg expression in the MS1096 domain. Increased wg expression by DΔNp53 is associated with tissue folding as visualized with actin staining (f) and with enhanced proliferation visualized with the PCNA-EmGFP reporter (i). Dp53 overproduction does not alter the overall Wg pattern but leads to an apparent thickening of the endogenous Wg domain (b). Dp53 overproduction does not induce tissue folding (e) and only induces some PCNA-EmGFP expression (h). Scale bars are 100 μm. (j) Quantification of EdU staining between the anterior and posterior compartments in control (en>p35) and wing discs overproducing Dp53 (en>Dp53) or DΔNp53 (en>DΔNp53). **P≤0.01, in Student's t-test