Figure 7.

EphA receptors are strongly tyrosine phosphorylated in mouse embryos overexpressing full-length ephrin-A5. Sagittal sections were prepared from E10.5 embryos overexpressing ephrin-A5 under the control of Wnt1-Cre, and then stained with anti-activated caspase-3 antibody (green) and anti-pTyr antibody (a–d), anti-pan p-EphA antibody (e–h), or anti-p-EphA4 antibody (i–l). Note that the apoptotic cells stained with anti-activated caspase-3 show irregular cell morphology and a high level of tyrosine-phosphorylated EphA receptors. Scale bars=500 μm (a) and 50 μm (b)