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RP-HPLC chromatograms of (A) nonradioactive (+)-IV-OH and (B) (+)-[125I] IV-OH after purification. Condition: flow rate was 1 mL/min. Mobile phase A was water with 0.1% triethylamine, and phase B was methanol with 0.1% triethylamine. The gradient conditions are as follows: 0 to 10 min, 70% to 80% B; 10 to 11 min, 80% to 100% B; and 11 to 20 min, 100% B.
