Fig. 2.

Distinct miRNA expression patterns during skeletal muscle injury and regeneration. A: number of miRNAs differentially expressed with >2-fold change during muscle regeneration compared with baseline, noninjured muscle. Data derived from 4 mice at each time point. B: hierarchical cluster analysis of differentially expressed miRNAs with >10-fold change during the time course of muscle injury and regeneration. Cluster analysis was performed for 86 differentially expressed miRNAs with >10-fold change after data adjustment (log transformation, median center and normalization). The color codes of red, black, and blue represent expression levels of high, average, and low, respectively. A–C, temporal expression of individual miRNA in cluster A (n = 10), cluster B (n = 43), and cluster C (n = 33) during muscle regeneration. The average value of miRNA expression in each cluster is presented as a red line; B, baseline, noninjured muscle. Data derived from 4 mice at each time point.