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. 2012 Sep 11;44(21):1042–1051. doi: 10.1152/physiolgenomics.00052.2012

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Fig. 5. — Effects of inhibition of miR-351 on apoptosis during MPC differentiation. MPC were subcultured and transfected with miRNA antisense (top) or scramble (bottom) oligonucleotide. Differentiation medium was added 1 day after transfection (differentiation day 0, Dif-D0). Caspase activity was measured at differentiation day 1 (Dif-D1) and labeled with a green fluorescence probe (FLICA; B and E); and nuclear integrity was monitored by Hoechst 33342 counterstaining (blue, A and D). C and F show merged images from green and blue fluorescence. Cell number was determined using images obtained with a fluorescent microscope to determine the nuclei/field (G) and the percent of caspase-positive cells (H). Data represent means ± SD of 3 different MPC cultures. *P < 0.001 compared with the scramble oligonucleotide.