Figure 7. DNA-PK contributes to the IRF-3-dependent innate immune response to MVA.
(A) Ifnb and Cxcl10 transcription was measured 6 hr following MVA infection of primary WT and Irf3−/− fibroblasts at an m.o.i. of 5. (B) Wild type and Prkdc−/− MEFs were infected with MVA or NDV and the level of induction of Cxcl10 and Il-6 were measured 6 hr later by qRT-PCR. (C) As (A) but with immortalised Xrcc5+/+/Trp53−/− and Xrcc5−/−/Trp53−/− MEFs. (D) The induction of Isg54 and Nfkbia mRNA was measured by qRT-PCR 6 hr after MVA or NDV infection of wild type and Prkdc−/− cells. (E) Expression of VACV proteins, analysed by immunoblotting with a rabbit polyclonal anti-VACV serum, at the indicated times following infection of primary Prkdc+/+ and Prkdc−/− MEFs with MVA (m.o.i. = 5). *** p<0.001, ** p<0.01, * p<0.05, n = 3, error bars ± SEM, ni; non-infected.