Figure 8. DNA-PKcs contributes to the innate immune response to MVA, HSV-1 and DNA in vivo.
(A) Groups of five Prkdc+/+ and Prkdc−/− mice were injected intradermally into the ear pinna with cationic lipids complexed with 1 μg DNA or poly(I:C). RNA was extracted from the tissue 12 hr later and Ifnb and Il6 transcription was measured by qPCR. (B) Groups of five Prkdc+/+ and Prkdc−/− mice were infected intradermally with 106 pfu of MVA or influenza virus strain A/PR/8/34 (PR8) and 12 hr later Ifnb and Il6 transcription was measured by qPCR from RNA extracted from the local site of infection. (C) As (B) but with HSV-1 strain S17. Note that levels of ifnb could not be measured above background in this experiment. *p<0.1, ** p<0.01, n = 5, error bars ± SEM, ni; non-infected.