Polyplex size (via dynamic light scattering) analysis of the end-capped polymers formulated at N/P = 7 (panel A) and 15 (panel B) in water, Opti-MEM (without serum), and DMEM containing 10% FBS (fetal bovine serum). Polyplexes with PEI were prepared at N/P = 5 and with Glycofect at N/P = 20. Polymer was added to plasmid DNA in water, incubated at room temperature for 1 hr to form polyplexes and then diluted into water, Opti-MEM, or DMEM immediately prior to measurement. Results represent the mean and standard deviation where n = 3.