Polyplex zeta potential analysis of end-capped polymers formulated at N/P = 7, 15, and 30 (adamantine-Tr4, 1A). Polymer was added to pDNA in water and incubated at room temperature for 1 hr, and diluted into additional water immediately prior to measurement, in the same manner as for particle size measurement. Results represent the mean and standard deviation where n = 3.