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Agarose gel electrophoresis shift assays to qualitatively assess the binding between the Tr4 polymer analogs and pDNA. Polymers were added to pDNA in water at the indicated N/P ratios and incubated at room temperature for 1 hr, followed by loading onto an ethidium bromide-containing 1% agarose gel and electrophoresis at 60 V for 40 min. A control sample (containing pDNA only) was run and is depicted as N/P = 0. All polymers exhibited complete pDNA binding at N/P ratios above 2.
