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. Author manuscript; available in PMC: 2013 Aug 13.
Published in final edited form as: Biomacromolecules. 2012 Jul 18;13(8):2229–2239. doi: 10.1021/bm300471n

Figure 6.

Figure 6

Flow cytometry analysis of Cy5-labeled luciferase plasmid uptake 4 hours after transfection. Polyplexes were formed in water and diluted into DMEM containing 10% FBS (panel A) or serum-free Opti-MEM (panel B) in the same manner as in particle size analysis (Fig. 3). Four hours after transfection, cells were trypsinized, extensively washed, and analyzed via flow cytometry. PEI polyplexes were formulated at N/P = 5, and Glycofect polyplexes were formulated at N/P = 20. The results are reported as the average intensity of 20,000 live cell events.