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MTT assay showing cell viability after exposure to the polyplex formulations. Polyplexes were formed in water and diluted into DMEM containing 10% FBS (panel A) or serum-free Opti-MEM (panel B) in the same manner as in particle size analysis (Fig. 3). PEI polyplexes were formulated at N/P = 5 and Glycofect polyplexes were formulated at N/P = 20. The data represents the mean and standard deviation of transfection (n=3), and were normalized to untransfected cells.
