Synaptic growth is not altered in the absence of Fife. A–D, Confocal z-projections of NMJ 6/7 (A, C) and NMJ 4 (B, D) colabeled with antibodies to Bruchpilot and HRP in wild-type (WT) (A, B) and fifeex/Df (fifeex1027/Df (3L) BSC412) (C, D) larvae. E, Quantification of the area of HRP signal at NMJ 4 of segment A3 shows no significant difference between wild type (n = 14 NMJs) and fifeex/ex (n = 19 NMJs) or fifeex/Df (n = 17 NMJs). Scale bars, 10 μm.