Skip to main content
. Author manuscript; available in PMC: 2013 Dec 10.
Published in final edited form as: Biomacromolecules. 2012 Nov 14;13(12):3990–4001. doi: 10.1021/bm301278f

Figure 7.

Figure 7

In vitro evaluations of CPMV-OG488E and eCPMV-OG488I. Representative confocal images depict uptake of CPMV-OG488E (A,C) and eCPMV-OG488I (B,D) by HeLa cells. Side panels in C and D are orthogonal sections from the respective images confirming internalization of VNPs. Both CPMV and eCPMV were tagged with OG488 (green), the cell membrane was stained with wheat germ agglutinin (WGA)-Alexa Fluor 555 (red), and the nucleus was stained with DAPI (blue). Flow cytometry was performed to measure cell uptake (E). Cells to the right of the vertical line were considered positive for A647, and the percent of positive cells for each sample was quantified (F). Unpaired asterisks denote statistical significance as compared to cells only control (* p < 0.05, *** p < 0.001). Difference in fluorescence intensity between eCPMV and CPMV formulations was also statistically significant (p < 0.05).