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Primers for CYP2E1 gene polymorphism
| Target codon | Sequence | Amplicon (bp) | Tm (°C) |
|---|---|---|---|
| CYP2E1* 5B | F5'-CCAGTCGAGTCTACATTGTCA-3' | 413 bp | 55 |
| RsaI | R5'-TTCATTCTGTCTTCTAACTGG-3' | ||
| CYP2E1 | F5'-GTGATGGAAGCCTGAAGAACA-3' | 729 bp for insertion | 66 |
| 96-bp insertion | R5'-CTTTGGTGGGGTGAGAACAG-3' | 633 bp for non-insertion |
Tm, melting temperature.
