Table 1.

Three different combinations of functional a priori information.

No a priori information	The ICG concentration and lifetime were reconstructed assuming homogeneous background optical properties. Approximate absorption coefficient and reduced scattering coefficient of animal tissue were used (${\mu }_a=0.01{\mathrm{mm}}^{-1}$ and ${\mu }_s^{\prime }=1.0{\mathrm{mm}}^{-1}$)
Born normalization	The DOT measurements were considered as intrinsic excitation measurements. The FT measurements were normalized using the corresponding excitation measurements. ICG concentration and lifetime maps were reconstructed assuming the optical properties of the rat were homogeneous. This method has been widely used to correct for background optical heterogeneity in FT. More details can be found in Ref. 33
DOT optical maps	Both absorption and scattering maps of the rats were reconstructed from the frequency domain DOT measurements. The reconstructed optical property maps were first used as an input for FT photon migration model. Then the ICG concentration and lifetime was reconstructed from FT measurements