The expression patterns of CXCL12 and CXCR4 in non-small cell lung cancer (NSCLC) cell lines. Total RNA and proteins were extracted from 5 cell lines and subjected to RT-PCR and western blot analysis, respectively, for CXCL12 and CXCR4. Both mRNA (A) and protein (B) expression of CXCR4 but not CXCL12 were observed consistently in 95D, 95C, H1975, and A549 cell lines. The stable CXCL12 transfectant (A549-CXCL12) was used as a CXCL12 positive-control cell line. Data are representative of 3 independent analyses.