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. 2012 Sep 12;32(37):12876–12884. doi: 10.1523/JNEUROSCI.2250-12.2012

Figure 1.

Figure 1.

The transient expression of Fgf20 occurs before sensory cell formation. A–C, Explants probed for Fgf20 mRNA shows very faint expression of Fgf20 at E12.5 that gradually increases until E14.5. Fgf20 is very faintly expressed at E12.5 (A) and strongly upregulated in E13.5 (B) and E14.5 explants (C). D–F Immunolabeling of Sox2 in E12.5-E14.5 explants also shows a gradual increase in Sox2 levels in the developing cochlea. Boxed areas in D–F are shown in higher magnification in D′–F′ with Sox2 (red) and Gfi1 (green) labeling at E12.5 to E14.5. G, RT-PCR analysis of cochlear tissue from various embryonic ages shows the relative expression of Sox2, Fgf20, and Atoh1.