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. 2012 Sep 12;32(37):12876–12884. doi: 10.1523/JNEUROSCI.2250-12.2012

Figure 7.

Figure 7.

Quantification of FGF20 rescue effects. A, Each cochlea was divided into four regions by length. Total number of hair cells (#HC) was counted in the apical 25% of the duct; control (CTL), n = 8; DAPT, n = 9; FGF20 + DAPT, n = 10. Controls consisted of 236 ± 38 hair cells in the apex; DAPT treatment decreased the total hair cell number to 24 ± 12 while adding FGF20-rescued total hair cell numbers to 146 ± 16 in the apex. S, Saccule. B, DAPT treatment truncated the cochlear duct. Duct lengths were measured from base to apex based on morphological features of the cochlea. The average length of cochlear ducts of control explants was 2143 μm ± 110 (SEM), while DAPT treatment significantly truncated the duct to 1836 μm ± 77 (SEM); CTL, n = 14; DAPT, n = 15; FGF20 + DAPT, n = 16. C, Hair cells were also counted in the apical 25% of the duct, taking into account the length of the duct, and we show the number of hair cells per 100 μm. Controls consisted of 44 ± 5 hair cells per 100 μm in the apex. DAPT treatment decreased this to 6 ± 2 hair cells, while Fgf20 rescued 33 ± 3 hair cells per 100 μm in the apex; CTL, n = 8; DAPT, n = 9; FGF20 + DAPT n = 10. D, The length of the sensory epithelium (SE), labeled by Sox2, was normalized to the length of the duct. In controls, Sox2 labeling spans the entire length of the duct, resulting in a value of 97.9% ± 0.6 (SEM). Upon DAPT treatment, only 46.0% ± 6.8 of the duct was spanned by Sox2 labeling. When FGF20 was supplemented, 84.8% ± 3.5 of the duct was spanned; CTL n = 14, DAPT n = 15, FGF20 + DAPT, n = 16. E, Since Sox2 was downregulated upon DAPT treatment, we measured the area of the sensory epithelium by quantifying the total area occupied by Sox2+ and Gfi+ cells. DAPT treatment also decreased the area of the sensory epithelium to 35% ± 9.5 (SEM) relative to control explants. Adding Fgf20 along with DAPT rescued the total area of the sensory epithelium to 69% ± 12 (SEM); CTL n = 14, DAPT n = 15, FGF20 + DAPT n = 16. Student's t test *p < 0.05, **p < 0.0005, ***p < 0.00005, n.s., Not significant.