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. 2012 Dec 10;7(12):e51415. doi: 10.1371/journal.pone.0051415

Figure 1. Identification of HYPK-interacting partners using pull-down coupled with MALDI-mass spectrometry.

Figure 1

Flow chart for pull-down experiment using purified 6HN-HYPK as bait (A); separation of pulled-down proteins by 1D SDS-PAGE using HeLa SCL as prey (B); 2D SDS-PAGE using STHdhQ111/HdhQ111 SCL as prey (C), 2D SDS-PAGE with STHdhQ7/HdhQ7 SCL as prey (D); probability based MOWSE score and parent MS and subsequent MS/MS spectra for HSP90AB1 (E). From the MOWSE score distribution, it is evident that the peptide mass fingerprint search identifying HSP90AB1 (MOWSE score 139) was significant (p≤0.05). MALDI spectra for 5 other proteins and details of MALDI-mass analyses for the identification of HYPK-interacting proteins are provided as Figures S1, S2, S3, S4, and S5 and Table S3 respectively.