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. 2012 Dec 10;7(12):e51415. doi: 10.1371/journal.pone.0051415

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© 2012 Choudhury et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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High molecular weight HYPK-complex formation by endogenous HYPK with EEF1A1 (AI) and HSPA1A/HSP70 (AII) in Neuro2A cells using native-PAGE western analysis; results obtained by similar experiments in STHdh^Q7/Hdh^Q7 and STHdh^Q111/Hdh^Q111 with LMNB2 (BI) and HTT (BII); result obtained by re-probing the blot shown in B with anti-HYPK antibody is shown in BIII, showing the difference in migration of purified HYPK and complex-bound HYPK; similar co-IP experiments in STHdh^Q7/Hdh^Q7 (C) along with Ponceau staining of the nitrocellulose membrane (CI). The blot is probed with anti-TP53 (CII) and anti-RELA (CIII) antibodies. In all the cases, anti-HYPK antibody was used to pull-down and the immunoprecipitate elute was analyzed to detect the interacting proteins. The loading wells in A and C are marked by arrows.