Figure 6. Effect of HYPK on recovery of heat denatured luciferase activity.

The in vivo chaperone activities of Neuro2A-U61 and Neuro2A HYPK-U61 cells in presence and absence of HYPK (before administration of heat shock) are compared (A). As mentioned in the Materials and Methods section, the cells were subjected to heat shock at 43°C for 1 h (HS) and re-incubated at 37°C CO₂ incubator for the next 6h (HS+R). In every case, heat shock was administered 24h post-transfection of Tet⁺Luc+ve cells with experimental constructs. Comparison of luciferase activities in Neuro2A-U61 cells in presence and absence of HYPK-DsRed in No HS, immediately after heat shock (HS) and HS+R conditions (B). These luciferase activities were also compared upon overexpression of HSPA8-DsRed and 3 other HYPK partners in Neuro2A-U61 cells (B). Such drop and recovery of the luciferase activities upon heat treatment of HYPK downregulated Neuro2A (Neuro2A-HYPK U61) cells and effect of HYPK and its interacting partners are shown (C). The ‘n’ and ‘p’ values for Student’s two-tailed t test are indicated in the bar diagrams along with the mean and standard deviation.