Table 1. HYPK-interacting proteins identified in the present study and obtained earlier.
| Gene identified (HUGO name) | HUGOsymbol | Gene ID (Human) | Source of protein | Validation method(s) |
| 1. Huntingtin | HTT | 3064 | –– | Faber et. al, Hum Mol Genet. 1998, vol. 7, 1463–74 |
| 2. Archain 1 | ARCN1 | 372 | SCL from HeLa (1D SDS-PAGE) | Not validated |
| 3. Eukaryotic translationelongation factor 1 alpha 1 | EEF1A1 | 1915 | SCL from HeLa (1D SDS-PAGE) | Confocal microscopy and co-IP, identified in more than one experiment |
| 4. Heat shock 70kDa protein 8 | HSPA8 | 3312 | SCL from HeLa and STHdhQ7/HdhQ7(Both 1Dand 2D SDS-PAGE) | Confocal microscopy and co-IP, identified in more than one experiment |
| 5. Lamin B2 | LMNB2 | 84823 | SCL from HeLa (1D SDS-PAGE) | Confocal microscopy and co-IP |
| 6. Lamin B receptor | LBR | 3930 | SCL from HeLa (1D SDS-PAGE) | Not validated |
| 7. Triosephosphate isomerase 1 | TPI1 | 7167 | SCL from HeLa (1D SDS-PAGE) | Not validated, identified in more than one experiment |
| 8. Serologically defined coloncancer antigen 1 | SDCCAG1 | 9147 | SCL from HeLa (1D SDS-PAGE) | Not validated |
| 9. Leukocyte receptor cluster(LRC) member 8 | LENG8 | 114823 | SCL from HeLa (1D SDS-PAGE) | Not validated |
| 10. IKBKB interacting protein | IKBIP | 121457 | SCL from HeLa (1D SDS-PAGE) | Not validated |
| 11. Calmodulin 1 | CALM1 | 801 | SCL from Neuro2A (1D SDS-PAGE) | Confocal microscopy and co-IP, identified in more than one experiment |
| 12. Cadherin 11 | CDH11 | 1009 | SCL from Neuro2A (1D SDS-PAGE) | Not validated |
| 13. Calreticulin | CALR | 811 | SCL from Neuro2A (1D SDS-PAGE) | Not validated |
| 14. Centrosomal protein290 kDa | CEP290 | 80184 | SCL from Neuro2A (1D SDS-PAGE) | Not validated, identified in more than one experiment |
| 15. Zinc finger protein 462 | ZNF462 | 58499 | SCL from Neuro2A (1D SDS-PAGE) | Not validated |
| 16. Zinc finger protein 100 | ZNF100 | 163227 | SCL from Neuro2A (1D SDS-PAGE) | Not validated |
| 17. Zinc finger protein 516 | ZNF516 | 9658 | SCL from Neuro2A (1D SDS-PAGE) | Not validated |
| 18. Kinesin family member 20B | KIF20B | 9585 | SCL from Neuro2A (1D SDS-PAGE) | Not validated |
| 19. Heat shock protein 90kDaalpha (cytosolic), class Bmember 1 | HSP90AB1 | 3326 | SCL from STHdhQ7/HdhQ7(1D SDS-PAGE) | Not validated, identified in more than one experiment |
| 20. Myomesin family, member 3 | MYOM3 | 127294 | SCL from STHdhQ7/HdhQ7(1D SDS-PAGE) | Not validated |
| 21. Phosphoglycerate mutase 1(brain) | PGAM1 | 5223 | SCL from STHdhQ111/HdhQ111 (1D SDS-PAGE) | Not validated |
| 22. NEDD4 binding protein 1 | N4BP1 | 9683 | SCL from Neuro2A (2D SDS-PAGE) | Not validated |
| 23. ATPase, H+ transporting,lysosomal V0 subunit a4 | ATP6V0A4 | 50617 | SCL from Neuro2A (2D SDS-PAGE) | Not validated |
| 24. Glutamate dehydrogenase 1 | GLUD1 | 2746 | SCL from Neuro2A (2D SDS-PAGE) | Not validated |
| 25. Glutamate dehydrogenase 2 | GLUD2 | 2747 | SCL from Neuro2A (2D SDS-PAGE) | Not validated |
| 26. Serrate RNA effector moleculeHomolog (Arabidopsis) | SRRT | 51593 | SCL from STHdhQ111/HdhQ111 (2D SDS-PAGE) | Not validated |
| 27. Protein phosphatase 6,regulatory subunit 2 | PPP6R2 | 9701 | SCL from STHdhQ111/HdhQ111 (2D SDS-PAGE) | Not validated |
| 28. Non-metastatic cells 2,protein (NM23B) | NME2 | 4831 | SCL from STHdhQ7/HdhQ7 (2D SDS-PAGE) | Not validated |
| 29. Vimentin | VIM | 7431 | –– | Confocal microscopy and co-IP |
| 30. Heat shock transcriptionfactor 1 | HSF1 | 3297 | –– | Confocal microscopy and co-IP |
| 31. Heat shock 70kDaprotein 1A | HSPA1A | 3303 | –– | Confocal microscopy and co-IP |
| 32. Heat shock 27kDa protein 1 | HSPB1 | 3315 | –– | Confocal microscopy and co-IP |
| 33. Myeloid leukemia factor 1 | MLF1 | 4291 | –– | Confocal microscopy and co-IP |
| 34. Myeloid leukemia factor 2 | MLF2 | 8079 | –– | Confocal microscopy and co-IP |
| 35. DnaJ (Hsp40) homolog, subfamily B, member 3 | DNAJB3 | 414061 | –– | Confocal microscopy and co-IP |
| 36. v-relreticuloendotheliosisviral oncogenehomolog A (avian) | RELA | 5970 | –– | Confocal microscopy and co-IP |
| 37. Tumor protein p53 | TP53 | 7157 | –– | Co-localization and co-IP |
| 38. N(alpha)-acetyltransferase 10, NatA catalytic subunit | NAA10 | 8260 | –– | Anderson et al., Molecular and Cellular Biology (2010), Vol. 30, No. 8, p. 1898–1909 |
| 39. N(alpha)-acetyltransferase 15, NatA auxiliary subunit | NAA15 | 80155 | –– | Same as above |
| 40. Heat shock 70kDa protein 14 | HSPA14 | 51182 | –– | Otto et al., PNAS, July 19, 2005, vol. 102, no. 29, p. 10064–10069 |
| 41. DnaJ (Hsp40) homolog, subfamily C, member 2 | DNAJC2 | 27000 | –– | Same as above |
| 42. Chromodomain helicase DNA binding protein 3 | CHD3 | 1107 | –– | Stelzl et al., 2005, Cell, Vol.122, 957–968 |
| 43. Group-specific component (vitamin D binding protein) | GC | 2638 | –– | Same as above |
| 44. MyoD family inhibitor | MDFI | 4188 | –– | Same as above |
| 45. Proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) | PSME3 | 10197 | –– | Same as above |
| 46. Quaking homolog, KH domain RNA binding (mouse) | QKI | 9444 | –– | Same as above |
| 47. RNA binding protein with multiple splicing | RBPMS | 11030 | –– | Same as above |
| 48. Rhoxhomeobox family, member 2 | RHOXF2 | 84528 | –– | Same as above |
| 49. TH1-like (Drosophila) | TH1L | 51497 | –– | Same as above |
HYPK-interacting proteins identified by us from pull-down of purified 6HN-HYPK protein as bait with various mammalian SCL as prey (no. 2–28), the HTT-interacting proteins as HYPK partners (no. 29–37) and previously reported HYPK-interacting partners (no. 1 and 38–49).