Table 1. Constitutive proteins secreted by Paracoccidioides Pb01 yeast and mycelia.
| General Information number (NCBI) 1 | Protein description | Number of isoforms in Paracoccidioides secretome 2 | Amount of isoform abundances 3 | ANOVA (p-value) 4 | SignalP Score ≥0.5 5 | SecretomeP Score ≥0.5 6 |
| 1. CELL RESCUE, DEFENSE and VIRULENCE | ||||||
| gi|295659787 | heat shock protein Hsp88 | 1 | 1.24 | 0.3645 | NO | NO |
| gi|295659837 | heat shock protein SSB1 | 1 | 2.77 | 0.0779 | NO | 0.8618 |
| gi|295669402 | Mn superoxide dismutase | 1 | 1.82 | 0.3780 | NO | 0.8823 |
| 2. METABOLISM | ||||||
| 2.1. Amino Acid Metabolism | ||||||
| gi|295667902 | aminomethyltransferase | 1 | 1.52 | 0.2302 | NO | 0.6367 |
| gi|295658698 | fumarylacetoacetase | 4 | 1.50 | 0.7047 | NO | 0.7492 |
| gi|295658947 | O-acetylhomoserine (thiol)-lyase | 1 | 1.00 | 0.9268 | NO | 0.7930 |
| gi|225683737 | spermidine synthase | 1 | 1.93 | 0.0864 | NO | NO |
| 2.2. Nucleotide Metabolism | ||||||
| gi|295674697 | adenosine kinase | 2 | 0.21 | 0.0646 | NO | NO |
| 2.3. Secundary Metabolism | ||||||
| gi|295666938 | nucleoside diphosphate kinase | 1 | 1.01 | 0.5725 | NO | NO |
| 2.4. Phosphate Metabolism | ||||||
| gi|295662360 | mannitol-1-phosphate 5-dehydrogenase | 3 | 0.42 | 0.0766 | NO | NO |
| 2.5. C-Compound and Carbohydrate Metabolism | ||||||
| gi|295667790 | beta-glucosidase | 1 | 1.63 | 0.2116 | 0.999 | 0.8279 |
| gi|295663469 | glycosyl hydrolase | 1 | 1.77 | 0.1350 | 0.996 | 0.8631 |
| gi|295665168 | TOS1 | 1 | 1.00 | 0.7772 | 0.999 | 0.9249 |
| 3. ENERGY | ||||||
| 3.1. Glycolysis and Gluconeogenesis | ||||||
| gi|295672732 | enolase | 4 | 0.67 | 0.0832 | NO | 0.5000 |
| gi|295671120 | fructose-bisphosphate aldolase | 5 | 398.84 | 0.0549 | NO | 0.6628 |
| gi|295658119 | glyceraldehyde-3-phosphate dehydrogenase | 2 | 0.76 | 0.4655 | NO | 0.9120 |
| gi|295669690 | phosphoglycerate kinase | 1 | 1.05 | 0.3639 | NO | 0.6626 |
| gi|225678203 | NmrA-like family protein | 1 | 1.01 | 0.5624 | NO | 0.5898 |
| 3.3. Tricarboxylic-acid Pathway | ||||||
| gi|295673937 | malate dehydrogenase | 1 | 1.36 | 0.0834 | 0.764 | 0.8163 |
| gi|295668473 | dihydrolipoyl dehydrogenase | 3 | 17.92 | 0.0541 | 0.571 | NO |
| 4. CELL CYCLE AND DNA PROCESSING | ||||||
| gi|295664474 | cell division cycle protein | 1 | 1.24 | 0.2116 | NO | NO |
| gi|295658863 | Cofilin/tropomyosin-type actin- binding family protein | 2 | 1.91 | 0.0093 | NO | NO |
| gi|295672736 | DNA damage checkpoint protein rad24 | 2 | 0.86 | 0.0693 | NO | NO |
| 5. PROTEIN FATE (folding, modification, destination) | ||||||
| gi|295663907 | peptidyl-prolyl cis-trans isomerase A2 | 1 | 1.06 | 0.4941 | 0.975 | 0.8963 |
| gi|295672668 | peptidyl-prolyl cis-trans isomerase B | 2 | 191.86 | 0.0920 | 0.928 | NO |
| gi|295662699 | peptidyl-prolyl cis-trans isomerase cypE | 1 | 1.12 | 0.2723 | NO | 0.8712 |
| gi|295672447 | peptidyl-prolyl cis-trans isomerase H | 3 | 144.1 | 0.0755 | NO | 0.7266 |
| gi|295665666 | Grp1p protein | 1 | 1.06 | 0.6021 | NO | 0.9061 |
| 6. UNCLASSIFIED PROTEINS | ||||||
| gi|295667926 | conserved protein | 1 | 1.01 | 0.7060 | 0.972 | 0.9280 |
| gi|295657286 | conserved protein | 1 | 1.00 | 0.7950 | NO | 0.8050 |
1
NCBI database general information number (http://www.ncbi.nlm.nih.gov/).
2
Number of identified isoforms of protein in Paracoccidioides, Pb01 secretome.
3
The average of amount of values of abundances of all identified isoforms used to statistical test.
4
ANOVA – statistically significant differences are considered with p<0.05 (*).
5
Secretion prediction according to Signal P 3.0 server, the number corresponds to signal peptide probability (http://www.cbs.dtu.dk/services/SignalP/).
6
Secretion prediction according to Secretome P 2.0 server, the number corresponds to neural network that exceeded a value of 0.5 (NN-score ≥0.50) (http://www.cbs.dtu.dk/services/SecretomeP/).