. 2012 Dec 18;7(12):e52470. doi: 10.1371/journal.pone.0052470

Table 2. Preferentially secreted proteins by Paracoccidioides Pb01 mycelia.

General Information number (NCBI) ¹	Protein description	Number of isoforms in mycelia ²	Amount of isoform abundances ³	ratio of M/Y abundance ⁴	ANOVA (p-value) ⁵	SignalP⁹Score ≥0.5 ⁶	SecretomeP Score ≥0.5 ⁷
1. CELL RESCUE, DEFENSE and VIRULENCE
gi\|295671569	heat shock protein SSC1	6	8.87	35.47	0.0171*	NO	0.6791
gi\|295670457	disulfide-isomerase tigA	1	0.07		**	NO	NO
gi\|295664022	glutathione reductase	4	0.87	4.98	0.0121*	NO	NO
gi\|225681400	peroxisomal catalase	2	0.28	61.29	0.0181*	NO	0.9389
gi\|295661107	thioredoxin reductase	1	1.47		**	NO	0.966
gi\|295656848	TCTP family protein	1	1.08		**	NO	NO
2. METABOLISM
2.1. Amino Acid Metabolism
gi\|295671621	choline dehydrogenase	1	0.09		**	NO	NO
gi\|295659538	Cobalamin-independent methionine synthase	1	0.04		**	NO	NO
gi\|295664250	histidine biosynthesis trifunctional protein	1	0.32		**	NO	0.6345
gi\|295661139	methylmalonate-semialdehyde dehydrogenase	2	0.41		**	NO	0.8078
gi\|295659992	serine hydroxymethyltransferase	1	0,15		**	NO	NO
2.2. Nucleotide Metabolism
gi\|295668873	phosphoribosylamine-glycine ligase	1	0.14		**	NO	NO
2.3. C-Compound and Carbohydrate Metabolism
gi\|295665123	aldehyde dehydrogenase	1	0.03		**	NO	NO
2.4. Nitrogen metabolism
gi\|295668479	formamidase	5	1.60	4.59	0.0033*	NO	NO
3. ENERGY
3.1. Glycolysis and Gluconeogenesis
gi\|295659988	2,3-bisphosphoglycerate -independent phosphoglycerate mutase	1	1.20		**	NO	0.6367
gi\|295670663	triosephosphate isomerase	1	1.52	2.52	0,0132*	NO	NO
3.2. Oxidation of fatty acids
gi\|295659859	acetyl-CoA acetyltransferase	2	0.29	6.47	0.0477*	NO	0.8393
gi\|295670934	electron transfer flavoprotein-ubiquinone oxidoreductase	1	0.10		**	NO	0.8751
3.3. Tricarboxylic-acid Pathway
gi\|295664721	aconitase	3	1.82	56.98	0.0064*	NO	0.6667
3.4. Pentose-phophate Pathway
gi\|295666688	transaldolase	2	0.53		**	NO	NO
gi\|295663567	6-phosphogluconolactonase	2	0.25	5.30	0.2191*	NO	0.6288
4. CELL CYCLE AND DNA PROCESSING
gi\|295658863	Cofilin/tropomyosin-type actin-binding family protein	2	18.754	1911.74	0.0076*	NO	NO
5. PROTEIN FATE (folding, modification, destination)
gi\|295672500	aminopeptidase	1	0.33		**	0.996	0.9401
gi\|295657201	glutamate carboxypeptidase	2	0.79	51.03	0.0199*	NO	0.7370
gi\|295658437	mitochondrial-processing peptidase subunit alpha	1	0.09		**	NO	0.6031
gi\|295668481	peptidyl-prolyl cis-trans isomerase D	1	0.28	10.46	0.0325*	NO	0.8640
6. PROTEIN SYNTHESIS
gi\|295674311	eukaryotic translation initiation factor 5A	1	0.38	5.15	0.0092*	NO	NO
gi\|295675019	elongation factor 2	1	2.54		**	NO	NO
gi\|295668925	elongation factor 1-gamma 1	1	0.19		**	NO	0.9067
7. UNCLASSIFIED PROTEINS
gi\|295658312	L-PSP endoribonuclease family protein	1	2.02		**	NO	NO

NCBI database general information number (http://www.ncbi.nlm.nih.gov/).

Number of identified isoforms of protein in Paracoccidioides, Pb01 mycelia phase secretome.

The average of amount of values of abundances of all identified isoforms used to statistical test.

⁴

The ratio of M to Y abundance in Paracoccidioides secretome.

⁵

ANOVA – statistically significant differences are considered with p<0.05 (*); proteins were not detected in yeast secretome (**).

⁶

Secretion prediction according to Signal P 3.0 server, the number corresponds to signal peptide probability (http://www.cbs.dtu.dk/services/SignalP/).

⁷

Secretion prediction according to Secretome P 2.0 server, the number corresponds to neural network that exceeded a value of 0.5 (NN-score ≥0.50) (http://www.cbs.dtu.dk/services/SecretomeP/).