. 2012 Dec 18;7(12):e52470. doi: 10.1371/journal.pone.0052470

Table 3. Preferentially secreted proteins by Paracoccidioides Pb01 yeast cells.

General Information number (NCBI) ¹	Protein description	Number of isoforms in yeast ²	Amount of isoform abundances ³	ratio of Y/M abundance ⁴	ANOVA (p-value) ⁵	SignalP Score ≥ 0.5 ⁶	SecretomeP Score ≥ 0.5 ⁷
1. CELL RESCUE, DEFENSE and VIRULENCE
gi\|295658865	heat shock protein 60	3	0.38	6.20	0.0121*	NO	NO
gi\|295665077	Hsp90 binding co-chaperone (Sba1)	2	1.33	20.97	0.0013*	NO	NO
gi\|295673162	disulfide isomerase Pdi1	1	0.20	5.76	0.0323*	0.988	0.899
gi\|295667577	glutathione S-transferase Gst3	1	2.44		**	NO	0.5119
gi\|295666684	Cu - Zn superoxide dismutase	2	0.46	2.72	0.0013*	NO	0.9184
gi\|295659831	thioredoxin-like protein	2	2.98	8.40	0.0394*	NO	0.8876
gi\|295659116	hsp70-like protein	6	596.89	7.91	0.0263*	NO	0.5000
2. METABOLISM
2.1. Amino Acid Metabolism
gi\|225680243	Cobalamin-independent methionine synthase	1	0.04		**	NO	NO
2.2. Secundary Metabolism
gi\|295663891	2,5-diketo-D-gluconic acid reductase A	1	0.09		**	NO	NO
3. ENERGY
3.1. Glycolysis and Gluconeogenesis
gi\|295662174	pyruvate kinase	1	0.09		**	NO	NO
3.2. Oxidation of fatty acids
gi\|295666179	2-methylcitrate synthase	8	645.67	70672.37	0.0039*	NO	0.5337
gi\|295662074	3-hydroxybutyryl-CoA dehydrogenase	1	1.79		**	NO	NO
gi\|295662032	enoyl-CoA hydratase	1	0.89		**	0.699	NO
4. CELL CYCLE AND DNA PROCESSING
gi\|295673184	actin-interacting protein	2	0.34	3.99	0.0018*	NO	0.9262
gi\|295661300	DNA damage checkpoint protein rad24	2	0.85		**	NO	NO
gi\|295667597	G4 quadruplex nucleic acid binding protein	1	1.96		**	NO	0.9401
gi\|295668188	nuclear movement protein nudC	1	0.24		**	NO	0.7668
gi\|295665468	nucleic acid-binding protein	2	18.32	19.49	0.0052*	0.982	0.5915
5. PROTEIN FATE (folding, modification, destination)
gi\|295660102	dipeptidyl-peptidase	3	0.27	3.47	0.0250*	NO	NO
gi\|295660961	gamma-glutamyltranspeptidase	1	1.26		**	NO	NO
gi\|295672926	proteasome component PRE4	1	0.77	15.82	0.0220*	NO	NO
6. CELLULAR TRANSPORT, TRANSPORT FACILITIES AND TRANSPORT ROUTES
gi\|295662829	vesicular-fusion protein SEC17	1	1.38		**	NO	NO
gi\|295660305	cytochrome-c oxidase chain VI	2	1.49		**	0.9240	NO
gi\|295670838	nucleolar transport factor 2	1	0.85		**	NO	0.9101

NCBI database general information number (http://www.ncbi.nlm.nih.gov/).

Number of identified isoforms of protein in Paracoccidioides, Pb01 yeast phase secretome.

The average of amount of values of abundances of all identified isoforms used to statistical test.

⁴

The ratio of Y to M abundance in Paracoccidioides secretome.

⁵

ANOVA - statistically significant differences are considered with p<0.05 (*); proteins were not detected in mycelia secretome (**).

⁶

Secretion prediction according to Signal P 3.0 server, the number corresponds to signal peptide probability (http://www.cbs.dtu.dk/services/SignalP/).

⁷

Secretion prediction according to Secretome P 2.0 server, the number corresponds to neural network that exceeded a value of 0.5 (NN-score ≥0.50) (http://www.cbs.dtu.dk/services/SecretomeP/).