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. 2012 Dec 18;7(12):e52188. doi: 10.1371/journal.pone.0052188

Figure 1. Drug-induced senescence is associated with secretion of Wnt ligands among other components of SASP.

Figure 1

Panel A. Doxorubicin-induced proliferation arrest was determined using melanoma cells 115 exposed to the drug at the indicated concentrations for 5 days and counted every day. The data represent average ±SE of three determinations. Panel B. Western blot showing the expression of cell cycle (p53, p21/WAF1 and cyclin D) and EMT genes (Zeb1, twist1, Vimentin and E-cadherin) in the absence and the presence of doxorubicin. Panel C. SA-beta Gal staining in control and cells treated 1 µM doxorubicin viewed by bright field microscopy. Panel D. mRNA expression levels of various growth factors, cytokines and Wnt ligands in response to cellular exposure to doxorubicin. Gene expression was normalized to that of GAPDH and represented in fold change compared to control non-treated cells. Each bar represents the average of three determinations ±SE. Statistical significance is shown for drug-treated cells versus control (*p<0.05, **p<0.001).