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. 2012 Dec 18;7(12):e51647. doi: 10.1371/journal.pone.0051647

Figure 3. rCD93D23 induces HUVEC migration through the activation of Akt and ERK.

Figure 3

(A) rCD93D23 dose-dependently induced FAK, ERK1/2, Akt, and eNOS phosphorylation. HUVECs were treated with different concentrations of rCD93D23 or VEGF (10 ng/ml) for 30 min. (B) rCD93D23 time-dependently induced FAK, ERK1/2, Akt, and eNOS phosphorylation in HUVECs. HUVECs were stimulated with 50 ng/ml of rCD93D23 for various time periods as indicated. The similar results were obtained in three independent experiments. *, p<0.05; **, p<0.01; ***, p<0.001 vs. control (C) HUVEC suspensions were pretreated with LY294002 (10 µM) or U0126 (10 µM). The lower wells were filled with rCD93D23 (30 ng/ml) or VEGF (10 ng/ml). Each value represents the mean ± SD (n = 3), and similar results were obtained in three different experiments. ***, p<0.001 vs. control. ###, p<0.001 vs. rCD93D23.