Figure 3. Frequency of PbCSP-specific CD8+ T-cells in different organs of BALB/c mice immunized with wt or spect (−) iSPZ.
BALB/c mice (4 mice per group) were immunized (iv) with wt or spect (−) ANKA PbiSPZ (one dose of 1×105 iSPZ). Seven (7) days later, PBL, liver and spleen cells of mice were isolated to determine frequency of PbCSP-specific CD8+ T-cells by flow cytometry (FACScan). The CD8+ T-cells were double stained with PE-conjugated PbCSP epitope tetramer and FITC-conjugated anti-mouse CD8b antibody. The naive group received neither wt nor spect (−) iSPZ. p-value compares statistically significant mean of CD8+ T-cell frequency between wt and spect (−) iSPZ-immunized groups.