Table 3. BALB/c mice injected with iSPZ-loaded hepatocytes are protected against SPZ challenge.

		2 weeks after challenge
Mouse group (n of SPZ for challenge)	Treatment	Mice protected/total challenged	% of protection	p-value: IS versus naive
A (2000)	IS	5/5	100	0.167
	Naive	2/5	40
B (5000)	IS	2/6	33	0.125
	Naive	0/10	0
Total	IS	7/11	64	0.014
	Naive	2/15	13

BALB/c mice were injected (IS transfer) with 7×10⁵ PbiSPZ-infected or naïve BALB/c hepatocytes. Infected hepatocytes were obtained from BALB/c mice immunized with 10⁶ ANKA wt iSPZ 2 h earlier. Recipient mice were then challenged with two different doses (2×10³ and 5×10³, respectively, A and B) of live PbSPZ one week later. Mice were considered protected if they remained parasite negative 2 weeks after challenge.