Table 4. Protection from infection in cyt c- and PBS- treated mice.

Treatment	Mice protected/total challenged	% of protection
Cyt c	10/10	100
PBS	10/10	100
Naive	1/10	10

Protection of mice after cyt c or PBS treatment from live SPZ challenge of two independent experiments (5 mice per group in each experiment). Mice were either pre-treated iv for 3 days with 5 mg/mouse of horse cyt c per day in 100 µl of PBS or with 100 µl of PBS alone. On the last day of treatment, mice were immunized iv with 1×10⁵ PbiSPZ in 100 µl of RPMI. One week later, mice were challenged with 5×10³ live PbSPZ. Parasitemia was checked at 1 and 2 weeks after challenge. Mice were considered protected if they remained parasite negative 2 weeks after challenge.