Figure 6. Vaccination Ag-loaded DCs boosts tumor protection in mice adoptively transferred with PKO, but not WT, CTLs. (A, B) WT CTL and PKO CTLs were generated as described in the legend to Figure 1 and transferred i.v. into naive recipients. One day later recipient mice were vaccinated s.c. with DC loaded with peptide Ags, DC loaded with protein Ags, or DC only, and challenged with tumor cells 5 d later. Tumor appearance was monitored every 2–3 d and mice were scored as tumor positive when tumor size was in excess of 4 mm². (A) Mice were injected with 5 × 10⁶ WT or PKO L318 CTLs and challenged with LL-LCMV tumors. The graph shows combined data from two independent experiments with a total of 13 mice/group. (B) Mice were injected with 1 × 10⁶ WT OT-I CTLs and challenged with B16-OVA tumor cells. The graph shows combined data from two independent experiments with a total of 10 mice/group. NS: not significant; **: 0.001 < p < 0.01 by a log-rank test.