Figure 1.CT-1^−/− mice are protected against the hepatic engraftment of MC38 colon cancer cells. (A) Individual size of MC38-derived tumors grafted in the liver for 14 d in wild type (WT), Il6^-/- and CT-1^−/− C57BL/6 mice. Mice were inoculated with 5 × 10⁵ MC38 colon carcinoma cells in the surgically exposed left lobe of the liver. Liver tumors were measured 14 d after tumor cell inoculation (**p < 0.01). (B) Experiment as in (A), showing the size of liver tumors in WT and CT-1^−/− mice backcrossed for two additional generations with selection for the most matched breeders by analyses of microsatellite polymorphism. (C) MicroCT images taken from representative mice per group on day 14 prior to surgery. (D) Littermate groups of mice of those shown in (A) were inoculated subcutaneously in the flank with 5 × 10⁶ MC38 cells. A sequential individual follow-up of the tumor mean diameter is shown.