Figure 6. Reduced number of MC38 cell-derived metastases from intrasplenic tumor cell inoculations in CT-1^−/− mice. (A) Representative hematoxylin and eosin (H&E)-stained tissue sections ( × 100 magnification) of the liver of wild type (WT) and CT-1^−/− littermates 14 d after the intrasplenic injection of 5 × 10⁶ MC38 cells. (B) Percentage of tissue area occupied by tumor nodules was quantified in slides stained with H&E and quantified using the Metamorph software.