Table 1. Primers.
Gene | Primers | |
Muc5ac (regular) | forward | CTGATAGTCACATACGAGGAGGG |
reverse | TCTTGGGACACTCTGTCTTCATT | |
Actin (regular) | forward | AGCCATGTACGTAGCCATCC |
reverse | TAGAAGCACTTGCGGTGCAC | |
Muc1 (realtime) | forward | TGGGGATCTCTAGCATCAAGTT |
reverse | GCTCCTACAAGTTGGCAGAAGT | |
Muc2 (realtime) | forward | GTGCTGCAATATCACCTCATGT |
reverse | TGTATGTGATGGAGCCTGAAAC | |
Muc4 (realtime) | forward | CGGGATTCCTTCTACGTTACAG |
reverse | GAATCGATCTGGACGGTACTTC | |
Muc5ac (realtime) | forward | CCATGCAGAGTCCTCAGAACAA |
reverse | TTACTGGAAAGGCCCAAGCA | |
Muc5b (realtime) | forward | GCTGCTGTTACTCCTGTGAAAAAG |
reverse | TGACCTCTGTCTCACAGCCCTTA | |
Actin(realtime) | forward | ACCGTGAAAAGATGACCCAGA |
reverse | GGAGTCCATCACAATGCCTGT |