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. 2012 Dec 18;7(12):e51911. doi: 10.1371/journal.pone.0051911

Figure 5. Effects of melatonin on APAP-induced release of cytochromec and AIF translocation.

Figure 5

Mice were treated as Materials and Methods. Liver samples were collected at 4 h after APAP administration. Nuclear translocation of AIF was analyzed using immunohistochemistry. Representative photomicrographs of liver histology from mice treated with saline (A as control), melatonin alone (B), APAP alone (C) and melatonin+APAP (D) are shown. Original magnification: 200×.(E) Cyt c in cytosol was detected by immunoblots. All experiments were repeated for four times. All data were expressed as means ± SEM (n = 4). **P<0.01 as compared with the control. ‡‡ P<0.01 as compared with APAP group.