Figure 1. hIL-2 is required for in vivo reconstitution of nT_Regs in NOD-scid IL2rγc^null mice.

(A) NOD-scid IL2rγc^null mice were hydrodymanically injected with human IL2 encoding plasmid and after 24h, their sera were tested for hIL2 by ELISA. (B) hIL-2 expressing or control NOD-scid IL2rγc^null mice were iv injected with 5×10⁶ in vitro expanded nT_Regs. On day 12 MNCs from spleen were analyzed for presence of human CD4⁺ CD25⁺ cells on human CD45 gated population. Each symbol represents an individual mouse and horizontal bars indicate the mean values. (C) 5×10⁶ CFSE labeled T_Regs were iv injected into hIL-2 expressing NOD-scid IL2rγc^null mice and spleen cells obtained 1 (M1) or 5 days (M2–M4) later were analyzed for CFSE dilution on human CD4 gated population.