Figure 4. In vivo expression of hIL-2 accelerates the induction of xenogenic GVHD in the presence or absence of T_reg reconstitution.

(A) Kaplan-Meir survival curve comparing IL-2 conditioned NOD-scid IL2rγc^null mice that received 5×10⁶ hPBMCs with or without prior reconstitution with 5×10⁶ T_Regs 12 days before injection with PBMCs. (B) IL-17A levels in the sera collected from the animals at the time of euthanasia. Error bars indicate standard deviation for three different animals tested. Supernatants from normal human PBMCs stimulated with PMA (50 ng/ml) and ionomycin (1 µg/ml) was used as positive control. (C) Kaplan-Meir analysis for survival of NOD-scid IL2rγc^null mice injected with graded doses of human PBMCs in presence of IL-2. Control and IL-2 conditioned animals were injected with different doses of hPBMCs (5×10⁶, 1×10⁶ and 0.5×10⁶) and observed for GVHD symptoms. Each group consisted of 3 mice.