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. 2012 Dec 18;7(12):e51804. doi: 10.1371/journal.pone.0051804

Figure 3. GST-δ-AmastinH specifically binds to HeLa cells in a dose-dependent saturable manner.

Figure 3

A. SDS-PAGE showing the purity of recombinant GST-δ-amastinH: 1; Molecular weigh markes, in kiloDaltons; 2: GST alone; 3: purified recombinant GST-δ-AmastinH; 4 total extract of induced E. coli. B: Recombinant GST-δ-AmastinH binds to HeLa cells. Increasing concentrations of GST-AmastinH or GST (negative control) were added to wells in ELISA plates containing adhered and fixed HeLa cells. After washing, cells were sequentially incubated with anti-GST antibodies and anti-rabbit IgG conjugated to peroxidase. The bound enzyme was revealed by o-phenylenediamine as a substrate. Representative results of two independent experiments are shown. *p<0.05.