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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: Oncogene. 2012 Sep 10;32(33):3877–3885. doi: 10.1038/onc.2012.392

Figure 2.

Figure 2

Regulation of Pdgfa is independent of BMP signaling through type I receptor. Cells were the treated with vehicle control medium or with 100 ng/ml BMP7 in the presence or absence of 10 μM dorsomorphin (DM) for 5h and 12h. Cells were harvested for total RNA and protein for real-time PCR and Western blotting. DM markedly reduced Id1 induction by BMP7 at 5h (A) and 12h (D), while no change is observed in Pdgfa expression (B and E). (C and F) Phosphorylation of SMAD1/5/8 in mouse granulosa cells detected by immunoblot after pretreatment with DM for 30 min followed by treatment with BMP7 for 5h and 12h. The membrane was stripped and re-probed for β-actin antibody for loading control. Different letters above the bars indicate statistical significance (n=3; P< 0.05).