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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: Oncogene. 2012 Sep 10;32(33):3877–3885. doi: 10.1038/onc.2012.392

Figure 4.

Figure 4

Loss of SMAD1/5 upregulates PDGFA in a human juvenile granulosa cell tumor-derived cell line (COV434). Real-time PCR was performed to compare the expression levels of SMAD1 (A) and SMAD5 (B), PDGFA (C) and PDGFB (D) in human embryonic kidney cell-line HEK293T (HEK) and human granulosa cell tumor derived cell line (COV434). Transfection of scrambled control siRNA (-KD) or siRNAs specific for SMAD1 and SMAD5 (+KD) resulted in a significant reduction of SMAD1 (E) and SMAD5 (F) in COV434 cells. Concomitantly, a significant 2-fold increase in PDGFA (G) is achieved following SMAD1 and SMAD5 reduction by siRNA, without a measurable change in PDGFB (H). Different letters above the bars indicate statistical significance (n=3; P< 0.05).