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. Author manuscript; available in PMC: 2013 Dec 14.
Published in final edited form as: Mol Cell. 2012 Oct 30;48(5):723–733. doi: 10.1016/j.molcel.2012.09.026

Figure 2. H2A.Z exchange mediates decreased nucleosome stability at DSBs.

Figure 2

(a) 293T cells expressing p400 or catalytically inactive p400ATPase, Tip60 or catalytically inactive Tip60HD were transfected with vector (Vec) or p84-ZFN (ZFN). 18hr post-transfection, cells were processed for ChIP using H2A.Z antibody, followed by RT-qPCR using primer pairs located 1.5kb to the right of the DSB. Results ± SD (n = 3). (b) 293T cells expressing a non-specific shRNA (shControl), shRNA targeting H2A.Z (shH2A.Z) or shH2A.Z expressing cells rescued by expression of an shRNA resistant HA-H2A.Z (HA-H2A.ZR) were incubated with bleomycin (5μM) for the indicated times (min). Cell pellets were extracted in 1.0M NaCl and released histones detected by western blot analysis for γH2AX, H2AX and H3. Ponceau S staining was used to demonstrate equal loading. See figure S3.