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. Author manuscript; available in PMC: 2013 Dec 14.
Published in final edited form as: Mol Cell. 2012 Oct 30;48(5):723–733. doi: 10.1016/j.molcel.2012.09.026

Figure 4. Histone H2A.Z is an essential component of the DNA damage response.

Figure 4

(a) 293T cells expressing a non-specific shRNA (○) or shRNA targeting H2A.Z (●) were irradiated and clonogenic cell survival measured. Results ± SD (n = 4). (b) 293T cells expressing a non-targeting shRNA (shCon) or shRNA targeting H2A.Z (shH2A.Z) were irradiated. 25hr later, cells were treated with colcemid, and the number of chromosome aberrations per cell counted. p was calculated using at t-test. (c) Cells with the stably integrated GFP-HR reporter system expressing a non-specific shRNA (shCon) or shRNA targeting H2A.Z (shH2A.Z) were transfected with the I-Sce1 nuclease to introduce DSBs, and GFP positive cells monitored by FACS. Results ± SD (n = 3). p-values calculated using a t-test. (d) Cells stably expressing an NHEJ-GFP reporter system were transfected with a non-specific vector (shCon) or shRNA targeting H2A.Z (shH2A.Z). Cells were transfected with the I-Sce1 nuclease and GFP positive cells measured. Results expressed ± SD (n = 3). p-values calculated using a t-test. See figure S5.